Customized polyclonal antibodies
We support your antibody production need through a wide range of customized protocols to produce high-performance antibodies. The polyclonal immune sera (without purification) or the purified polyclonal antibodies (IgG fraction, anti-idiotypic fraction,…). could be provided for diverse applications such as ELISA, Western Blotting, Immunostaining, Immunoprecipitation, Flow Cytometry…
The require quantity of immunogen to produce polyclonal antibodies is between 800-1000 µg per rabbit. This amount depends on the immunization program or the antigen’s nature.
For your project and your required applications we provide a wide range of additional services such as: antigen synthesis, ELISA control, purification, labeling,…
If you would like to learn more about our immunization program’s options please contact us.
FOCUS: What is polyclonal antibody?
Immunoglobulins or antibodies are heavy plasma’s proteins, carrying sugar chains on their amino acids residues (N-link glycosylation or O-linked glycosylation). Antibodies protect the body from harmful pathogens. After the recognition of the antigen by the immune system, B cells differentiate into memory B cells and plasma cells. The plasma cells then produce the specific antibodies of antigen. Several B cells produce a heterogeneous mixture of antibodies, each one targeting a different epitope on the same antigen.
The production of polyclonal antibodies through rabbit immunization is a time- and cost-effective approach, which produces large quantities of high specific antibodies with a high affinity.
FOCUS: Which antigen suits your program?
The choice of antigen is the key success factor of the project. Its available quantity, purity and antigenicity determine the magnitude of the animal immune response. Your antigen of interest can be analyzed by our experts to determine its immunogenicity and homology; depending on the selected species and/or any other molecule of interest.
AGRO-BIO: Pioneer in the customized production of polyclonal antibodies
Agro-Bio is one of the first service provider companies which offer high-quality customized antibody production programs since 1980. The first antibody production in Agro-Bio was carried out through immunization of chicken by protein injection, followed by the extraction of specific IgY antibodies from egg yolk.
In the wake of the success of our immunization projects and high-level request, the immunization of other animal species such as rabbit, mouse, rat, guinea pig, and goat was developed.
Nowadays, Agro-Bio has an accredited multi-species production unit that provides a safe and optimal environment for its personnel and for the success of its client’s projects; an immunotechnology research laboratory equipped with the newest laboratory platforms (Biacore T200, AKTA, SARTOFLOW,…); and a highly qualified scientific team that assist you over all phases of your project.